Part:BBa_K3456000:Design
I1-60GFPHis - ATPase Inhibitor with GFP and His-Tag
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We designed the part with a 17x helix linker between the I1-60 and the eGFP to add some space between these basic parts. It remains to be tested if the linker has any influence on the functionality.
Source
The original IF1 amino acid sequence is: MAATALAART RQAVWSVWAM QGRGFGSESG DNVRSSAGAV RDAGGAFGKR EQAEEERYFR ARAKEQLAAL KKHHENEISH HAKEIERLQK EIERHKQSIK KLKQSEDDD
It originates from Bos taurus (Bovine). UniProtKB - P01096 (ATIF1_BOVIN)
References
The procedure for the affinity purification was first mentioned in the paper "The affinity purification and characterization of ATP synthase complexes from mitochondria" (Runswick Michael J., Bason John V., Montgomery Martin G., Robinson Graham C., Fearnley Ian M. and Walker John E. 2013 The affinity purification and characterization of ATP synthase complexes from mitochondria Open Biol.3120160 http://doi.org/10.1098/rsob.120160).